Body Fluid Cytology

Requirements:

  • Container: clean sterile container securely capped for transport to SRL Cytology department
  • Specimen: submit fresh (unfixed) fluid. Transport immediately after collection to SRL Cytology department. If the specimen cannot be transported immediately the specimen must be:
    • Refrigerated: may be kept 24–48 hours
  • Storage: refrigerate Performed: Monday – Friday
  • Reported: Two business days
  • Reference Range: Narrative report
  • Method: Microscopy  

Breast Nipple Discharge Cytology

Requirement:

  • Container: Slide folder/carrier
  • Specimen: One or more nipple discharge smears; spray fix immediately
  • Storage: Room temperature
  • Performed: Monday - Friday
  • Reported: Two business days
  • Reference range: Narrative report
  • Methodology: Microscopy

Bronchial Alveolar Lavage Cytology

Requirements:

  • Container: clean sterile container
  • Specimen: submit fresh (unfixed) specimens. Transport immediately after collection to SRL Cytology department. Note: If cell count is needed, submit to hematology for differential count
  • Storage: refrigerate
  • Performed: Monday – Friday
  • Reported: Two business days
  • Reference range: Narrative report
  • Method: Microscopy

Bronchial Cytology

Requirements:

  • Container: clean sterile large enough to allow addition of fixative.
  • Specimen: submit fresh (unfixed) bronchial wash, brush, tracheal aspirate, Wang Needle biopsy immediately after collection to the SRL Cytology department. If the specimen cannot be transported immediately the specimen must be:
    • Refrigerated: may be kept 12–24 hours.
  • Storage: refrigerate.
  • Performed: Monday – Friday
  • Reported: Two business days.
  • Reference Range: Narrative report.
  • Methodology: Microscopy.

Cerebrospinal Fluid Cytology

Requirements:

  • Container: One CSF tube
  • Specimen: 2 ml CSF (minimum: 1 ml) submit fresh (unfixed) fluid. Transport immediately after collection to SRL Cytology department
  • Performed: Monday – Saturday
  • Reported: Two business days
  • Reference range: Narrative report
  • Methodology: Microscopy

Cyst Fluid Cytology

Requirements:

  • Container: Cytolyt vial Specimen: submit cyst fluid in 30ml Cytolyt vial
  • Storage: room temperature
  • Patient information: Identify anatomic site
  • Performed: Monday – Friday
  • Reported: Two business days
  • Reference range: Narrative report
  • Methodology: Microscopy

Cytomegalic Inclusion Disease (CMV) Cytology

Test includes screening for CMV plus routine cytology.

Requirements:

  • Container: Clean sterile container large enough to allow addition of fixative
  • Specimen: submit 20-50 ml of fresh (unfixed) urine, sputum, or bronchial samples (min. 5ml) and transport immediately to SRL Cytology department. If the specimen cannot be transported immediately the specimen must be:
    • Refrigerated; or preserved in 30ml Cytolyt solution. Note: Do not use this method to preserve the specimen when other tests such as culture must be performed from the same container
  • Storage: refrigerate
  • Patient information: Give clinical history and diagnosis, including immuno-compromised status
  • Performed: Monday – Saturday
  • Reported: Two business days
  • Reference range: Narrative report
  • Methodology: Microscopy

Esophageal Cytology

Requirements:

  • Container: Clean container large enough to allow for the addition of fixative
  • Specimen: submit 20–30 ml of fresh (unfixed) esophageal wash (min. 10ml) and/or brushing; transport immediately to SRL Cytology department. If the specimen cannot be transported immediately the specimen must be refrigerated
  • Patient Information: include anatomic site, clinical history and diagnosis
  • Performed: Monday – Friday
  • Reported: Two business days
  • Reference range: Narrative report
  • Methodology: Microscopy

Fine Needle Aspiration (FNA) Cytology

Requirements:

If specimen is a cystic lesion; see Cyst Fluid cytology. If a solid lesion, call lab for complete procedure.

Pneumocystis Carinii Cytology

Test includes: Screening of GMS (silver) stain plus routine cytology.

Requirements:

  • Container: Clean container large enough to allow addition of fixative
  • Specimen: submit fresh (unfixed) 20-30 ml of washing or lavage. Bronchial alveolar lavage, bronchial wash/brush, tracheal aspirate, sputum (least valuable). Send specimen immediately to SRL Cytology department. If the specimen cannot be transported immediately the specimen must be:
    • Refrigerated; or preserved in 30ml of Cytolyt solution. Note: Do not use this method to preserve the specimen when other tests must be performed from the container
  • Storage: refrigerate
  • Performed: Monday – Friday
  • Reported: Two business days
  • Reference range: Narrative report
  • Methodology: Microscopy  

Sputum Cytology

Requirements:

  • Container: clean sterile container large enough to allow addition of fixative
  • Specimen: submit fresh (unfixed) Sputum; transport immediately to SRL Cytology department. If the specimen cannot be transported immediately; the specimen must be:
    • Refrigerated; or preserved in 30ml Cytolyt solution. Do not use this method to preserve the specimen when other tests must be performed from the container
  • Storage: refrigerate
  • Performed: Monday – Friday
  • Reported: Two business days
  • Reference range: Narrative report
  • Methodology: Microscopy    

Tzanck Smear Cytology (including Thin Prep Method)

Requirements:

  • Container: Slide folder/carrier for direct smears. PreservCyt solution vial for liquid based Thin Prep
  • Specimen: Direct Smear; submit one to three slides prepared by opening a fresh blister, and when possible make two smears from the evacuated fluid by spreading thinly across the glass slides. After collecting the fluid sample, carefully use a blade or spatula (do not use swabs) to scrape the base of the blister; then smear the collected material thinly across the glass slide. The smears are to be fixed immediately with Cytology spray fixative. If you have any questions on how to properly collect the specimen, please call the Cytology department at 517.364.2010 or 517.364.3651
  • Storage: room temperature
  • Specimen: Alternate Thin Prep method: submit specimen in PreservCyt solution. To separate the specimen from the collection device, vigorously rinse the brush/scraper by swirling in the preservative and brushing against the wall of the collection vial. When this action is complete discard the brush/scraper, tightly cap the vial, label the vial with the patients name, and source of specimen. Transport specimen to the SRL Cytology department
  • Storage: room temperature or refrigerated
  • Patient information: anatomic site
  • Performed: Monday – Friday
  • Reported: Two business days
  • Reference range: Narrative report
  • Methodology: Microscopy  

Urine Cytology

Requirements

  • Container: clean sterile container large enough to allow addition of fixative
  • Specimen: submit 20–50ml (min. 10ml) of fresh (unfixed) urine and transport immediately to SRL Cytology department. If the specimen cannot be transported immediately the specimen must be:
    • Refrigerated or preserved in 30ml of Cytolyt solution. Note: Do not use this method to preserve the specimen when other tests must be performed from the container
  • Storage: refrigerate
  • Performed: Monday – Friday
  • Reported: Two business days
  • Reference range: Narrative report
  • Methodology: Microscopy