- Sample should be pancervical representing 360° of endo/ectocervix.
- Specimen must be immediately placed in the PreservCyt vial and rinsed properly.
- Specimen must represent an adequate number of cells.
Supplies Needed:
Speculum, gloves, plastic spatula/endocervical brush or broom-like device, cotton swabs, PreservCyt solution vial, cytology requisition, and
specimen bag.
ThinPrep Collection Procedure using the Endocervical Brush/Spatula:
- Obtain supplies.
- Insert the speculum. Cleanse surface of ectocervix with cotton swab, removing the mucus and debris.
- Introduce the plastic spatula tongue into the cervical canal, so that it’s "shoulder" rests at the ectocervix.
- Rotate the spatula 360° using slight, but constant pressure so that it always remains in contact with ecto and endocervical mucosa (transitional zone).
- Rinse the spatula as quickly as possible into the PreservCyt solution vial by swirling the spatula vigorously in the vial 10 times.
- Obtain an adequate sampling from the endocervix using an endocervical brush device. Insert the brush into the cervix
until only the bottom most fibers are exposed. Slowly rotate ¼ or ½ turn in one direction. DO NOT OVER-ROTATE.
- Rinse the brush as quickly as possible in the PreservCyt Solution by rotating the device in the solution 10 times
while pushing against the PreservCyt vial wall. Swirl the brush vigorously to further release material. Discard the brush.
- Tighten the cap so that the torque line on the cap passes the torque line on the vial.
- Record the patient’s name on the vial and fill in all patient information and medical history on the cytology requisition form.
- Place the vial and requisition in a specimen bag for transport to the laboratory.
ThinPrep Collection Procedure using the Broom-like Device:
- Obtain supplies.
- Insert the speculum. Cleanse surface of ectocervix with cotton swab, removing mucus and debris.
- Obtain an adequate sampling from the cervix using a broom-like device. Insert the central bristles of the broom into
the endocervical canal deep enough to allow the shorter bristles to fully contact the ectocervix. Push gently, and rotate the broom in a clockwise direction five times.
- Rinse the broom as quickly as possible into the PreservCyt Solution vial by pushing the broom into the bottom of the
vial 10 times, forcing the bristles apart. As a final step, swirl the broom vigorously to further release material. Discard the collection device.
- Tighten the cap so that the torque line on the cap passes the torque line on the vial.
- Record the patient’s name on the vial and fill in the patient information and medical history on the cytology requisition form.
- Place the vial and requisition in a specimen bag for transport to the laboratory. Reference: Part No. 85217-001 Rev. E, 2000, Cytyc Corporation
Note: Cytyc Corporation has provided SRL with a Quick Reference guide that gives a visual representation of the sample collection technique. If you would like a copy of the Reference Guide
please contact the Cytology department at 517.364.2010.
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